Module Functional Analysis
Figure 1: Functional Analysis Menu and Side Panel
Introduction
The Functional Analysis Module is a comprehensive bioinformatics tool for functional annotation and analysis of sequence data.
The module uses the Blast2GO methodology to extract the GO terms associated with the obtained hits and returns an evaluated GO annotation for the query sequence(s). Enzyme codes are obtained by mapping from equivalent GOs while InterPro motifs are directly queried at the InterProScan web service. GO annotation can be visualized by reconstructing the structure of the Gene Ontology relationships and pathways.
A typical basic use case of the Functional Analysis Module consists of 5 steps: BLASTing, mapping, annotation, statistical analysis, and visualization. These steps will be explained in the next sections.
Additional Resources
Functional Annotation Analysis use case: https://www.biobam.com/whole-genome-functional-annotation-of-solanum-lycopersicum/ .
Functional Analysis Example Dataset: https://resources.biobam.com/omicsbox/example_data/version_2_0_0/FunctionalAnalysis.zip